Date: Tuesday, May 20, 2014
Speaker: Brian Strahl, University of North Carolina at Chapel Hill
Title: Decoding the Role of Histone Modifications in Chromatin Regulation
Place: 110 Willamette Hall
Time: 4:00 – 5:00 p.m.
Host: Eric Selker
Our laboratory aims to understand how histone post-translational modifications contribute to the structure and function of chromatin. A diverse number of histone modifications have been identified, and are thought to work in a combinatorial manner to drive the distinct functions found in chromatin (e.g., gene transcription and DNA repair). However, how histone modifications work together in the context of a ‘histone code’ to regulate chromatin function is still poorly understood. To address this issue, our lab recently developed a high-throughput peptide microarray platform, where hundreds of synthetic histone peptides that are combinatorially modified with distinct histone modifications are arrayed on glass slides. With this technology, we have been interrogating a wide number of chromatin-associated proteins that “write” and/or “read” the histone code. One such protein we have recently focused on is UHRF1, an E3 ubiquitin ligase essential for DNA methylation. We have found that this protein binds to H3 lysine 9 methylation to regulate the maintenance of DNA methylation in human cells, and further, that multiple histone-interaction domains in this protein "read" a specific histone signature on H3 to do so. These studies define how histones, as well as their modifications, drive fundamental epigenetic processes in the cell.
|Tue May 20, 2014 4pm – 5pm Pacific Time|
|110 Willamette Hall (map)|